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Classic Glass Preparative TLC PLC Plates Silica Gel 60 F254 1 or 2 mm Layer Thickness (1000 or 2000 μm/microns) 20 x 20 cm Size 8 or 6 Plates/Box AAdvance Instruments (2 mm, 6)
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About the item
- Classic Preparative TLC Plates. Silica Gel 60 F254 type. 20x20cm plate size, 2mm (2000μm) silica layer thickness. pH 6.2-6.8, particle size 10-40 μm, pore size 60 Å. 6 plates per box. Glass backed. A test run separating dimethyl maleate and dimethyl fumerate has been performed and shown in photos. 50mg of each compound (total 100mg), dissolved in a small a of hexane, acetone and DCM was spotted onto plate. The plate was eluted twice with hexane:acetone=15:1, giving a very good separation. While this is expected, this also indicated that depending on how far away each component band is on TLC, one can load material as of 200mg (for the 2 mm) and more onto the 2mm plate since in the 1mm example the plates have not quite reached full capacity. (demo used 1mm plate of same specs.) The preparative TLC method is an alternative to preparative HPLC. HPLC methods can be more expensive and when handled improperly, contamination can occur. Prep TLC is especially suitable for applications when there is relatively a small a of sample, and purity requirements are high, and when at the same time the researcher is looking for a quick, convenient and economic way of purification..
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